Chicken IDO(Indoleamine-2,3-Dioxygenase) ELISA Kit

• Catalog Number: orb1088191
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Indoleamine-2,3-Dioxygenase(IDO). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Indoleamine-2,3-Dioxygenase(IDO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Indoleamine-2,3-Dioxygenase(IDO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Indoleamine-2,3-Dioxygenase(IDO) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Gallus
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• Protein Length: 3.5h
• Alternative names: CD107B, INDO, Indoleamine-Pyrrole 2,3 Dioxygenase
• Note: For research use only
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Expiration Date: Please enquire.