Chicken DAO(Diamine Oxidase) ELISA Kit

• Catalog Number: orb1146909
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diamine Oxidase(DAO). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Diamine Oxidase(DAO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diamine Oxidase(DAO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diamine Oxidase(DAO) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Gallus
• Range: 0.63-40 ng/mL
• Sensitivity: 0.236 ng/mL
• Protein Length: 3.5h
• Alternative names: AOC1, ABP1, KAO, Histaminase, Kidney amine oxidase
• Note: For research use only
• Sample Types: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Expiration Date: Please enquire.