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Chicken CalcitoninGene Related Peptide 1 (CGRP1) ELISA Kit

Catalog Number: orb1146908

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1146908
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Calcitonin (CT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Calcitonin (CT) . Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin (CT) in the samples is then determined by comparing the OD of the samples to the standard curve.
ReactivityGallus
Range15.63-1000 pg/mL
Assay Time3.5h
Concentration1000 pg/mL
Sensitivity4.98 pg/mL
Alternative namesCALCA, CALC1, CGRP-I, CGRP1, KC, CCP, PDN-21, Kata
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NoteFor research use only
Application notesstandard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken CGRP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken CGRP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken CGRP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken CGRP1 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration DatePlease enquire.
Chicken CalcitoninGene Related Peptide 1 (CGRP1) ELISA Kit