CHIA antibody

• Catalog Number: orb341111
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CHIA
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human AMCase
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CHIA
• Entrez: 27159, 81600, 113901
• UniProt ID: Q6RY07, Q91XA9, Q9BZP6
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Acidic mammalian chitinase; AMCase; Lung-specific
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of AMCase expression in HepG2 (A), mouse stomach (B), rat lung (C) whole cell lysates. (Predicted band size: 33; 40; 52 kD; Observed band size: 52 kD)

Immunohistochemical analysis of AMCase staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.