CHD3 antibody

• Catalog Number: orb1474229
• Category: Antibodies
• Description: Mouse monoclonal antibody to CHD3
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli.
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Conjugation: Unconjugated
• Target: CHD3
• Entrez: 1107
• UniProt ID: Q12873
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: Chromodomain-helicase-DNA-binding protein 3; CHD-3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CHD3 expression in NTERA2 (A), NIH3T3 (B), C6 (C), K562 (D), Hela (E) whole cell lysates. (Predicted band size: 227 kD; Observed band size: 260 kD)

Immunofluorescent analysis of CHD3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.