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Catalog Number | orb20494 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to CHCHD3 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Rat, Sheep |
Dilution range | ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml |
Conjugation | Unconjugated |
MW | 26.2 |
Target | CHCHD3 |
Entrez | 54927 |
Protein Sequence | KFKRYESHPVCAD |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti coiled-coil-helix-coiled-coil-helix domain co Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 26kDa band observed in Human Brain (Cerebellum) and Heart lysates (calculated MW of 26.2kDa according to NP_060282.1). Recommended concentration: 1-3 μg/ml.Anonymous customer finds ~30kDa in THP1 and in human primary monocyte derived dendritic cells. |
Expiration Date | 12 months from date of receipt. |
orb20494 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical staining of Human Heart using CHCHD3 antibody
Immunohistochemical staining of Human Skeletal Muscle using CHCHD3 antibody
ICC, IF, IP, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Canine, Mouse, Rat, Sheep | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
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