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Catalog Number | orb1474634 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to CHAT |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | Recombinant fusion protein of human CHAT. The exact sequence is proprietary. |
Dilution range | WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | CHAT |
Entrez | 1103 |
UniProt ID | P32738, Q03059, P28329 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | Choline O-acetyltransferase; CHOACTase; ChAT; Chol Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of CHAT expression in HepG2 (A), HeLa (B), C6 (C) whole cell lysates. (Predicted band size: 70 kD; Observed band size: 70 kD)
Immunofluorescent analysis of Integrin alpha 7 staining in rat brain. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
WB | |
Animal, Bovine, Canine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish | |
Canine, Equine, Guinea pig, Human, Mouse, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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