Chat Antibody

• Catalog Number: orb1247994
• Category: Antibodies
• Description: Chat Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-KERARGPTKPSNLD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3kDa according to Mouse NP_034021.1 and Rat NP_001164064.1). Recommended concentration:0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Frozen sections of Mouse Forebrain shows staining of neural cell bodies. Optimal dilution: 1:2000.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: Chat
• UniProt ID: Q03059
• NCBI: NP_034021.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Chat, choline acetyltransferase , B230380D24Rik
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3kDa according to Mouse NP_034021.1 and Rat NP_001164064.1). Recommended concentration:0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Frozen sections of Mouse Forebrain shows staining of neural cell bodies. Optimal dilution: 1:2000.
• Expiration Date: 12 months from date of receipt.

orb1247994 (1:2000) staining of PFA-perfused cryosection of Mouse Forebrain. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method.