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Catalog Number | orb692675 |
---|---|
Category | Antibodies |
Description | CH25H antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Anti-CH25H antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of human CH25H conjugated to Keyhole Limpet Hemocyanin (KLH). |
Concentration | 0.92 mg/ml |
Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 1:100, WB: 1:1,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This affinity purified antibody is directed against human CH25H. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence and 92% homology to pig, 91% homology to rat, and 75% homology to mouse. |
Conjugation | Unconjugated |
UniProt ID | O95992 |
NCBI | NP_003947.1 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Rabbit Anti-Cholesterol 25-Hydroxylase, Rabbit Ant Read more... |
Note | For research use only |
Application notes | Anti-CH25H Antibody has been tested in ELISA, WB, and IHC. Expect a band at ~31.7kDa in western blot using appropriate tissues or lysates. Positive control used: Human Kidney Tissues in Immunohistochemistry. |
Expiration Date | 12 months from date of receipt. |
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