You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb546357 |
---|---|
Category | Antibodies |
Description | ch TOG/CKAP5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human ch TOG/CKAP5 recombinant protein (Position: M1-L221). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 225 kDa |
UniProt ID | Q14008 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Cytoskeleton-associated protein 5; Colonic and hep Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U937 cells using anti-ch TOG/CKAP5 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of ch TOG/CKAP5 using anti-ch TOG/CKAP5 antibody.Lane 1:human HeLa cell;2:human HepG2 cell;3:human MCF-7 cell;4:human U87 cell;5:rat brain tissue;6:mouse brain tissue.
IF analysis of ch TOG/CKAP5 using anti-ch TOG/CKAP5 antibody. ch TOG/CKAP5 was detected in an immunocytochemical section of A431 cells.
IHC analysis of ch TOG/CKAP5 using anti-ch TOG/CKAP5 antibody. ch TOG/CKAP5 was detected in a paraffin-embedded section of human liver cancer tissue.
IHC analysis of ch TOG/CKAP5 using anti-ch TOG/CKAP5 antibody. ch TOG/CKAP5 was detected in a paraffin-embedded section of human prostate cancer tissue.
IHC analysis of ch TOG/CKAP5 using anti-ch TOG/CKAP5 antibody. ch TOG/CKAP5 was detected in a paraffin-embedded section of mouse brain tissue.
IHC analysis of ch TOG/CKAP5 using anti-ch TOG/CKAP5 antibody. ch TOG/CKAP5 was detected in a paraffin-embedded section of rat brain tissue.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
Filter by Rating