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Catalog Number | orb1806554 |
---|---|
Category | Assays and Kits |
Description | cGMP(Cyclic Guanosine Monophosphate) ELISA Kit |
Reactivity | All |
Range | 156.25-10000 pg/mL |
Target | cGMP |
Sensitivity | 45.2 pg/mL |
Alternative names | c-GMP; 3',5'-Cyclic GMP; Cyclic 3',5'-GMP; Guanosi Read more... |
Note | For research use only |
Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cyclic Guanosine Monophosphate(cGMP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cyclic Guanosine Monophosphate(cGMP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclic Guanosine Monophosphate(cGMP) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |
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