CGB Antibody

• Catalog Number: orb1252784
• Category: Antibodies
• Description: CGB Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HCGb/54
• Tested applications: ELISA, IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant protein was used as the immunogen for this HCG-beta antibody.
• Concentration: 0.2 mg/mL
• Dilution range: ELISA: use BSA-free format (1)IHC (FFPE): 0.5-1 ug/ml for 30 min at RT (2)Prediluted format : incubate for 30 min at RT (3)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCG-beta antibody to be titered up or down for optimal performance.1. Clone HCGb/54 (recommended detect) will pair with clones HCGb/211 (recommended capture) and HCGb/459 by ELISA.2. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CGB
• UniProt ID: Q6NT52
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: CGB, CGB3, CG-beta, Choriogonadotropin beta chain,
• Note: For research use only
• Application notes: ELISA: use BSA-free format (1)IHC (FFPE): 0.5-1 ug/ml for 30 min at RT (2)Prediluted format : incubate for 30 min at RT (3)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCG-beta antibody to be titered up or down for optimal performance.1. Clone HCGb/54 (recommended detect) will pair with clones HCGb/211 (recommended capture) and HCGb/459 by ELISA.2. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of placenta stained with HCG-beta antibody (HCGb/54). Note specific membrane staining.