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Catalog Number | orb1252642 |
---|---|
Category | Antibodies |
Description | CGB Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | HCGb/459 |
Tested applications | ELISA, IHC |
Reactivity | Human |
Isotype | IgG1, kappa |
Immunogen | Recombinant protein was used as the immunogen for this HCG-beta antibody. |
Concentration | 0.2 mg/mL |
Dilution range | ELISA: use BSA-free format (2)IHC (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (3)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCG-beta antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. Clone HCGb/459 (recommended capture) will pair with clones HCGb/211 and HCGb/54 (recommended detect) by ELISA.3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CGB |
UniProt ID | Q6NT52 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | CGB, CGB3, CG-beta, Choriogonadotropin beta chain, Read more... |
Note | For research use only |
Application notes | ELISA: use BSA-free format (2)IHC (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (3)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCG-beta antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. Clone HCGb/459 (recommended capture) will pair with clones HCGb/211 and HCGb/54 (recommended detect) by ELISA.3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical testing of human placenta with HCG-beta antibody (HCGb/459).
ELISA, IHC-P, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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