CGA Mouse Monoclonal Antibody

SKU: orb10782

Description

CGA Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 4A8
• Immunogen: Natural human hCG protein
• Target: CGA
• Molecular Weight: 10 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS (pH7.4) with 0.02% Proclin300.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

GLHA_HUMAN; Glycoprotein hormones alpha chain; Anterior pituitary glycoprotein hormones common subunit alpha; HCG; LHA; FSHA; GPA1; GPHa; TSHA; GPHA1; Choriogonadotropin alpha chain; Chorionic gonadotrophin subunit alpha(CG-alpha); Follicle-stimulating hormone alpha chain(FSH-alpha); Follitropin alpha chain; Luteinizing hormone alpha chain(LSH-alpha); Lutropin alpha chain; Thyroid-stimulating hormone alpha chain(TSH-alpha); Thyrotropin alpha chain; glycoprotein hormones, alpha polypeptide; HCG alpha;

(Negative control) Paraformaldehyde-fixed, paraffin embedded Human Pancreas, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CGA Monoclonal Antibody, Unconjugated (orb10782) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Measured by its binding ability in a indirect ELISA. Immobilized Human HCG alpha protein, HisTag at 2 µg/mL (100 µl/well) can bind Mouse Anti-Human HCG alpha Antibody, the EC50 is 40.93 ng/ml.

Paraformaldehyde-fixed, paraffin embedded Human pituitary gland, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CGA Monoclonal Antibody, Unconjugated (orb10782) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Placenta, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CGA Monoclonal Antibody, Unconjugated (orb10782) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Quick Database Links

Gene Symbol

CGA

UniProt

P01215