CFC1 Antibody

• Catalog Number: orb1247646
• Category: Antibodies
• Description: CFC1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Human, Rat
• Reactivity: Mouse
• Immunogen: The immunogen for this antibody is: HFTGRYCEHDQRRSEC
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 55kDa band in Human Breast, Placenta and Testis lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.6kDa according to NP_115934.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Mouse Embryonic Liver shows staining of the hepatocyte cell membranes. Recommended concentration: 1-2ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CFC1
• UniProt ID: P0CG37
• NCBI: NP_115934.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CFC1, HTX2, CRYPTIC, cripto, FRL-1, cryptic family
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 55kDa band in Human Breast, Placenta and Testis lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.6kDa according to NP_115934.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Mouse Embryonic Liver shows staining of the hepatocyte cell membranes. Recommended concentration: 1-2ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247646 (2 ug/ml) staining of paraffin embedded Mouse Embryo Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.