CES1 antibody

• Catalog Number: orb19790
• Category: Antibodies
• Description: Goat polyclonal antibody to CES1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:8000, WB: 0.03-0.1 μg/ml, IHC-P: 2-3µg/ml
• Conjugation: Unconjugated
• MW: 62.6; 62.5; 62.4
• Target: CES1
• Entrez: 1066
• Protein Sequence: NTQAAQKLKDKE
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CES1 antibody, anti carboxylesterase 1 (monoc
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Lung shows staining of lymphocytes in the vasculature.. Recommended concentration, 2-3ug/ml.WB: Approx 55-60kDa band observed in Human Liver and Ileum lysates (calculated MW of 62.6kDa according to NP_001020366.1). In transfected HEK293 transiently expressing CES1 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19790 (0.03ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19790 (2.5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.