CEP290 Antibody

• Catalog Number: orb1528008
• Category: Antibodies
• Description: CEP290 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IP, WB
• Reactivity: Human
• Immunogen: between 2429 and 2479
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; ICC-IF - 1:250 - 1:1,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CEP290
• UniProt ID: O15078
• NCBI: NP_079390.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: Bardet-Biedl syndrome 14 protein; monoclonal antib
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CEP290 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CEP290 antibody orb1528008 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. CEP290 was also immunoprecipitated by rabbit anti-CEP290 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 second (B).

Detection of human CEP290 by immunocytochemistry.Sample: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CEP290 (Cat. No. orb1528008) used at a dilution of 1: 500 (2 µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 used at a dilution of 1: 100.