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Catalog Number | orb1928039 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Target | This CENPH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-162 amino acids from the Central region of human CENPH. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
UniProt ID | Q9H3R5 |
MW | 28481 Da |
Tested applications | FC, IHC-P, WB |
Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Antibody Type | Primary Antibody |
Clone Number | RB22481 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Centromere protein H, CENP-H, Interphase centromer Read more... |
Note | For research use only |
NCBI | NP_075060.1 |
Western blot analysis of CENPH Antibody (Center) in Hela cell line lysates (35 ug/lane). CENPH (arrow) was detected using the purified Pab.
CENPH Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human testis tissue reacted with CENPH Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.