CENP-F/Mitosin Antibody

• Catalog Number: orb1528072
• Category: Antibodies
• Description: CENP-F/Mitosin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 2350 and 2400
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CENP-F/Mitosin
• UniProt ID: P49454
• NCBI: AAA82889.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: CENP-F kinetochore protein; STROMS; PRO1779; mitos
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CENP-F/Mitosin by immunohistochemistry. Sample: FFPE section of human ewing sarcoma. Antibody: Affinity purified rabbit anti-CENP-F/Mitosin used at a dilution of 1:200 (1µg/ml).

Detection of human CENP-F/Mitosin by WB and IP.Samples: HeLa and HEK293T cells. Antibodies: Affinity purified rabbit anti-CENP-F/Mitosin antibody orb1528072 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate.