CENP-A antibody

• Catalog Number: orb148101
• Category: Antibodies
• Description: Mouse monoclonal to CENP-A (ATTO-488). A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5A7-2E11
• Tested applications: ICC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Synthetic peptide corresponding to a portion of human CENP-A
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Conjugation: ATTO-488
• MW: 18kDa
• Target: CENPA
• Entrez: 1058
• UniProt ID: P49450
• NCBI: NP_001035891.1
• Storage: Conjugated antibodies should be stored at 4°C
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Alternative names: CENP A antibody, cenpa antibody, Centromere auto a
• Note: For research use only
• Application notes: 1 µg/ml of SMC-202 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.