CELF2 Antibody / CUGBP2 / ETR3

• Catalog Number: orb2636589
• Category: Antibodies
• Description: Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-CELF2-1E4
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant full-length human CELF2/CUGBP2 protein was used as the immunogen for the CELF2 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml)
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CELF2 antibody is available for research use only.
• UniProt ID: O95319
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CELF2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells using CELF2 antibody (green) and phalloidin (red).

Immunofluorescent staining of PFA-fixed human MCF-7 cells using CELF2 antibody (green) and phalloidin (red).

Immunofluorescent staining of human U-87 cells using CELF2 antibody (green) and phalloidin (red).

FACS staining of PFA-fixed human MCF7 cells with CELF2 antibody (blue) and unstained cells (red).

FACS staining of PFA-fixed human HeLa cells with CELF2 antibody (blue) and unstained cells (red).

FACS staining of PFA-fixed human U-87 cells with CELF2 antibody (blue) and unstained cells (red).

SDS-PAGE analysis of purified, BSA-free CELF2 antibody as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CELF2 antibody (clone PCRP-CELF2-1E4). These results demonstrate the foremost specificity of the PCRP-CELF2-1E4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.