CEBP Beta/CEBPB Antibody

• Catalog Number: orb215925
• Category: Antibodies
• Description: CEBP Beta/CEBPB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CEBP Beta recombinant protein (Position: M1-A200). Human CEBP Beta shares 61% amino acid (aa) sequence identity with both mouse and rat CEBP Beta.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 36106 MW
• UniProt ID: P17676
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: CCAAT/enhancer-binding protein beta ;C/EBP beta ;L
• Note: For research use only
• Application notes: WB: The detection limit for CEBP Beta is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of CEBP Beta using anti-CEBP Beta antibody.Lane 1:human HeLa cell;2:human Caco-2 cell.

WB analysis of CEBP Beta using anti-CEBP Beta antibody.Lane 1:rat liver tissue;2:rat kidney tissue;3:mouse liver tissue;4:mouse kidney tissue;5:human HeLa cell.

IF analysis of CEBP Beta using anti-CEBP Beta antibody. CEBP Beta was detected in an immunocytochemical section of U20S cells.

IHC analysis of CEBP Beta using anti-CEBP Beta antibody. CEBP Beta was detected in a paraffin-embedded section of human placenta tissue.