C/EBP beta Antibody

• Catalog Number: orb1526084
• Category: Antibodies
• Description: C/EBP beta Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 50 and 100
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: C/EBP beta
• UniProt ID: P17676
• NCBI: NP_005185.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: CCAAT/enhancer binding protein (C/EBP), beta; Live
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human C/EBP beta by western blot. Samples: Whole cell lysate from HeLa (50, 15, 5 µg) , HEK293T (50 µg) , and Jurkat (50 µg) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody orb1526084 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human C/EBP beta by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody orb1526084 used for IP at 6 µg per reaction.

Detection of human C/EBP beta by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-C/EBP beta (Cat. No. orb1526084) used at a dilution of 1: 1, 000 (1µg/ml) Detection: DAB