C/EBP alpha (phospho-Ser21) antibody

• Catalog Number: orb764343
• Category: Antibodies
• Description: Rabbit polyclonal antibody to C/EBP alpha (phospho-Ser21)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human C/EBP-alpha around the phosphorylation site of Ser21. AA range:6-55
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 45
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-CEBPA antibody, anti-CCAAT/enhancer-binding p
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from Mouse-liver, Rat-liver, Hela cells treated with 0.01U/ml 30min, using C/EBP α was diluted at 1:10000, 37° 1hour.

Western Blot analysis of various cells using Phospho-C/EBP α (S21) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using C/EBP-alpha (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide.