CEACAM5 Antibody

• Catalog Number: orb1252640
• Category: Antibodies
• Description: CEACAM5 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C66/1030
• Tested applications: ELISA, FC, IF, IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Human full-length recombinant CEA protein was used as the immunogen for this antibody.
• Concentration: 0.2 mg/mL
• Dilution range: ELISA: order BSA-free format (3)Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. Clone C66/1030 (recommended capture) will pair with clone C66/1009 (recommended detect) by ELISA. It will NOT pair with clone C66/261.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CEACAM5
• UniProt ID: P06731
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: CEACAM5 Antibody : CEA, CD66e, CEA, Carcinoembryon
• Note: For research use only
• Application notes: ELISA: order BSA-free format (3)Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.; 3. Clone C66/1030 (recommended capture) will pair with clone C66/1009 (recommended detect) by ELISA. It will NOT pair with clone C66/261.
• Expiration Date: 12 months from date of receipt.

FFPE staining of human colon carcinoma with CEA antibody C66/1030.