CEA (C3) Mouse Monoclonal Antibody

• Catalog Number: orb10367
• Category: Antibodies
• Description: CEA (C3) Mouse Monoclonal Antibody
• Target: CEACAM5
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M PBS (pH7.4) with 0.02% Proclin300.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human CEA/CD66e/CEACAM5 full length
• UniProt ID: P06731
• RRID: AB_10751931
• MW: 150-200 kDa
• Tested applications: ELISA, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-50javascript:;0, IF=1:100-500, ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Clone Number: C3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Carcinoembryonic antigen; Carcinoembryonic antigen
• Research Area: Cancer Research, Molecular Biology, Surface Molecu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human liver cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CEA (C3)) Polyclonal Antibody, Unconjugated (orb10367) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.