Cdk4 Antibody

• Catalog Number: orb308830
• Category: Antibodies
• Description: Cdk4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Cdk4 recombinant protein (Position: G201-E303). Human Cdk4 shares 94.2% amino acid (aa) sequence identity with both mouse and rat Cdk4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 33730 MW
• UniProt ID: P11802
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cyclin-dependent kinase 4;2.7.11.22;Cell division
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of CDK4 using anti-CDK4 antibody (Left) and anti-CDK4 antibody (Right).Lane 1:human HeLa cell; 2:human K562 cell; 3:rat PC-12 cell; 4:mosue NIH/3T3 cell; 5:mosue RAW264.7 cell.

IF analysis of CDK4 using anti-CDK4 antibody. CDK4 was detected in a paraffin-embedded section of human colon cancer tissue.

IHC analysis of Cdk4 using anti-Cdk4 antibody. Cdk4 was detected in a paraffin-embedded section of human lung cancer tissue.

IHC analysis of Cdk4 using anti-Cdk4 antibody. Cdk4 was detected in a paraffin-embedded section of mouse intestine tissue.

IHC analysis of Cdk4 using anti-Cdk4 antibody. Cdk4 was detected in a paraffin-embedded section of rat intestine tissue.