CDK1 Antibody

• Catalog Number: orb308828
• Category: Antibodies
• Description: CDK1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CDK1 recombinant protein (Position: L66-M297). Human CDK1 shares 97.8% and 98.3% amino acid (aa) sequence identity with mouse and rat CDK1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 34095 MW
• UniProt ID: P06493
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cyclin-dependent kinase 1;CDK1;2.7.11.22;2.7.11.23
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U937 cells using anti-CDK1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CDK1 using anti-CDK1 antibody.Lane 1:Rat Thymus Tissue;2:Rat Spleen Tissue;3:MCF-7 Cell;4:HELA Cell;5:JURKAT Cell;6:NIH3T3 Cell.

IF analysis of CDK1 using anti-CDK1 antibody.CDK1 was detected in immunocytochemical section of U20S cell.

IHC analysis of CDK1 using anti-CDK1 antibody. CDK1 was detected in a paraffin-embedded section of human mammary cancer tissue.

IHC analysis of CDK1 using anti-CDK1 antibody. CDK1 was detected in a paraffin-embedded section of rat testis tissue.

IHC analysis of CDK1 using anti-CDK1 antibody. CDK1 was detected in a paraffin-embedded section of mouse testis tissue.