CDH5 (phospho-Y731) antibody

• Catalog Number: orb515190
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CDH5 (phospho-Y731)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y731 of human CD144 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:50-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CDH5
• Entrez: 1003, 12562
• UniProt ID: P55284, P33151
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Cadherin-5; 7B4 antigen; Vascular endothelial cadh
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD144 (Phospho-Y731) expression in Hela Starved 4h (A), Hela Starved 16h (B), Hela Starved 24h (C) whole cell lysates. (Predicted band size: 87 kD; Observed band size: 130 kD)

Immunohistochemical analysis of CD144 (Phospho-Y731) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.