CDH4 Antibody

• Catalog Number: orb1268616
• Category: Antibodies
• Description: CDH4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This CDH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-203 amino acids from the N-terminal region of human CDH4.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 100 kDa
• Target: CDH4
• UniProt ID: P55283
• NCBI: P55283
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Cadherin-4, Retinal cadherin, R-CAD, R-cadherin, C
• Note: For research use only
• Application notes: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

Formalin-fixed and paraffin-embedded human brain tissue reacted with CDH4 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Immunofluorescence analysis of CDH4 Antibody Antibody with paraffin-embedded human brain tissue. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

Flow cytometric analysis of HepG2 cells using CDH4 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.