CDH2 Antibody

• Catalog Number: orb1252210
• Category: Antibodies
• Description: CDH2 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 8C11
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1, kappa
• Immunogen: A human partial recombinant protein corresponding to the N-Cadherin extracellular domain was used as the immunogen for this CDH2 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CDH2 antibody to be titered up or down for optimal performance.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CDH2
• UniProt ID: P19022
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: CDH2, CDw325, CDHN, Cadherin-2, CD325, CD325 antig
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CDH2 antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human brain lysate with CDH2 antibody. Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).

IHC testing of FFPE mouse heart tissue with CDH2 antibody. Required HIER: boil tissue sections in 10mM Tis with pH 9 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.