CDH11 antibody

• Catalog Number: orb20653
• Category: Antibodies
• Description: Goat polyclonal antibody to CDH11
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Canine, Human, Rat
• Dilution range: ELISA: 1:8000, WB: 0.1-0.5 μg/ml
• Conjugation: Unconjugated
• MW: 88
• Target: CDH11 (aa 497-509)
• Entrez: 1009
• Protein Sequence: CESDQTKPLSNQP
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CDH11 antibody, anti cadherin 11, type 2, OB-
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: In transfected HEK293 transiently expressing full-length Human CDH11 (myc and DYKDDDDK tagged), a band of approx. 130kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.1-0.5 μg/ml.Experiment Notes: Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.
• Expiration Date: 12 months from date of receipt.

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CDH11 with C-terminal MYC tag probed with orb20653 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with orb20653 (1mg/ml) in Lane B.