CDC73 Antibody

• Catalog Number: orb1262332
• Category: Antibodies
• Description: CDC73 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Gallus, Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This CDC73 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132-161 amino acids from the Central region of human CDC73.
• Concentration: batch dependent
• Dilution range: For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 61 kDa
• Target: CDC73
• UniProt ID: Q6P1J9
• NCBI: Q6P1J9
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Parafibromin, Cell division cycle protein 73 homol
• Note: For research use only
• Application notes: For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Overlay histogram showing U-2OS cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Overlay histogram showing U-2OS cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Antibody staining CDC73 in human adrenal gland tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Antibody staining CDC73 in human adrenal gland tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: TT whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of CDC73 Antibody in 293 cell line lysates (35 ug/lane)

Confocal immunofluorescent analysis of CDC73 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).