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Catalog Number | orb670294 |
---|---|
Category | Antibodies |
Description | CDC27 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human CDC27 recombinant protein (Position: H609-F824). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.25-0.5μg/ml, Human, Mouse Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 92 kDa |
UniProt ID | P30260 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of human Raji whole cell lysates (Lane 1), human Jurkat whole cell lysates (Lane 2), human Jurkat whole cell lysates(Lane 3), mouse thymus tissue lysates(Lane 4) using CDC27 antibody.
Flow cytometric analysis of A431 cells using CDC27 antibody.
Immunohistochemical staining of human bladder cancer tissue using CDC27 antibody.
Immunohistochemical staining of human gastric cancer tissue using CDC27 antibody.
Immunohistochemical staining of human melanoma tissue using CDC27 antibody.
Immunohistochemical staining of human rectal cancer tissue using CDC27 antibody.
Immunohistochemical staining of human skin cancer tissue using CDC27 antibody.
Immunohistochemical staining of mouse intestine tissue using CDC27 antibody.
Immunohistochemical staining of rat intestine tissue using CDC27 antibody.
Immunofluorescence analysis of A431 cells using CDC27 antibody.
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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