CD98 Antibody [RL388], Rabbit IgG

• Catalog Number: orb1671702
• Category: Antibodies
• Description: CD98 Antibody [RL388], Rabbit IgG
• Clonality: Recombinant
• Clone Number: RL388
• Tested applications: FC, IP, Stim
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising rats with an IL 2-producing subline of the mouse T lymphoma EL4.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Slc3a2
• UniProt ID: P10852
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: 4F2hc, 4F2 cell-surface antigen heavy chain, Solut
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of mouse splenocytes using anti-CD98 antibody (RL388). Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of RL388 (orb1671702) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671702- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD98 antibody RL388. Mouse fetal liver lysate (35 ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric r version of RL388 (orb1671702) at 0.05 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.