CD97 monoclonal antibody (M01), clone 5D5

• Catalog Number: orb2294721
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a partial recombinant CD97.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5D5
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG2a Kappa
• Immunogen: CD97 (NP_510966, 421 a.a. ~ 529 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: KKQAELEEIYESSIRGVQLRRLSAVNSIFLSHNNTKELNSPILFAFSHLESSDGEAGRDPPAKDVMPGPRQELLCAFWKSDSDRGGHWATEGCQVLGSKNGSTTCQCSH
• NCBI: NP_510966
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged CD97 is approximately 0.1 ng/ml as a capture antibody.

Western Blot analysis of CD97 expression in transfected 293T cell line by CD97 monoclonal antibody (M01), clone 5D5. Lane 1: CD97 transfected lysate(81.7 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of CD97 over-expressed 293 cell line, cotransfected with CD97 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CD97 monoclonal antibody (M01), clone 5D5 (Cat # orb2294721). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (37.73 KDa).