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Catalog Number | orb1733234 |
---|---|
Category | Antibodies |
Description | CD94 Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | residues 32-179 (ECD) |
Concentration | 250 µg/ml |
Dilution range | WB: 1:1000, IP: 20 µl/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | CD94 |
UniProt ID | Q13241 |
Storage | Shelf life: 1 year from date of receipt. Storage: 2 - 8°C |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | Killer cell lectin-like receptor subfamily D membe Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human CD94 by western blot. Samples: Whole cell lysate (10 µg) from Jurkat, NK-92, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD94 recombinant monoclonal antibody (orb1733234) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human CD94 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from NK-92 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD94 recombinant monoclonal antibody (orb1733234) used for IP at 20 µl/mg lysate.
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