CD9 Antibody

• Catalog Number: orb371664
• Category: Antibodies
• Description: CD9 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived mouse CD9 recombinant protein (Position: T110-I193). Mouse CD9 shares 77.4% and 86.9% amino acid (aa) sequence identity with human and rat CD9, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse Flow Cytometry, 1-3 μg/1x106 cells, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 25258 MW
• UniProt ID: P40240
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: CD9 antigen;CD9;Cd9;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of RAW264.7 cells using anti-CD9 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of CD9 using anti-CD9 antibody.Lane 1:mouse kidney tissue.

WB analysis of CD9 using anti-CD9 antibody.Lane 1:rat thymus tissue; 2:rat lung tissue; 3:mouse spleen tissue; 4:mouse lung tissue; 5:mouse HEPA1-6 cell.

IF analysis of CD9 using anti-CD9 antibody. CD9 was detected in immunocytochemical section of HEPA1-6 cells.

IHC analysis of CD9 using anti-CD9 antibody. CD9 was detected in paraffin-embedded section of mouse intestine tissue.