CD9 antibody (Biotin)

• Catalog Number: orb154465
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to Biotin which recognizes CD9 that is amember of the tetraspanin (TM4SF) family. It expressed by a variety of hematopoietic and epithelial cells. It involved in platelet activation, cell adhesion, cell motility and tumor metastasis and aggregation and also regulates paranodal junction formation.
• Clonality: Monoclonal
• Clone Number: EM-04
• Tested applications: FC, ICC, IP, WB
• Reactivity: Mouse
• Isotype: Rat IgG1
• Immunogen: Permeabilized murine bone marrow-derived mast cells (BMMC).
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 2-4 μg/ml.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: CD9
• Entrez: 12527
• UniProt ID: P40240
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Mic3 antibody, anti-Mrp-1 antibody, anti-Btcc
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 2-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining pattern of murine splenocyte suspension stained using anti-mouse CD9 (EM-04) Biotin antibody (concentration in sample 2 μg/ml, Streptavidin APC) and SYTO16.

Separation of murine myeloid cells (red-filled) from CD9 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD9 (EM-04) Biotin antibody (concentration in sample 2 μg/ml, Streptavidin APC).

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD9 (EM-04) Biotin antibody (concentration in sample 2 μg/ml, Streptavidin APC).