CD8b antibody (PE )

• Catalog Number: orb154509
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to PE which recognizes CD8B that also known as P37 and LEU2. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system.
• Clonality: Monoclonal
• Clone Number: 341
• Tested applications: FC
• Reactivity: Rat
• Isotype: Mouse IgG1 kappa
• Immunogen: CD8 positive Wistar rat splenic T cell hybridomas
• Concentration: 0.5 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD8b
• Entrez: 926
• UniProt ID: P10966
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Ly-3 antibody, anti-Ly-C antibody, anti-Lyt-3
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of rat CD8b positive cells (red-filled) from CD8b negative cells (black-dashed) in flow cytometry analysis (surface staining) rat splenocyte suspension stained using anti-rat CD8b (341) PE antibody (concentration in sample 3 μg/ml).

Flow cytometry surface staining pattern of rat splenocyte suspension stained using anti-rat CD8b (341) PE antibody (concentration in sample 3 μg/ml).