CD86 antibody (FITC)

• Catalog Number: orb44818
• Category: Antibodies
• Description: Rat Monoclonal to CD86.
• Clonality: Monoclonal
• Clone Number: GL-1
• Tested applications: FC
• Reactivity: Mouse
• Isotype: Rat IgG2a kappa
• Immunogen: LPS-activated CBA/Cs mouse splenic B cells
• Concentration: 0.5 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-2 μg/ml; positive control: murine splenocytes.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD86
• Entrez: 12524
• UniProt ID: P42082
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD86 (mouse) antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-2 μg/ml; positive control: murine splenocytes.
• Expiration Date: 12 months from date of receipt.

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD86 (GL-1) FITC antibody (concentration in sample 0.33 μg/ml).

Separation of murine CD86 positive myeloid cells (red-filled) from CD86 negative lymphoid cells (black-dashed) in flow cytometry analysis (surface staining) murine splenocyte suspension stained using anti-mouse CD86 (GL-1) FITC antibody (concentration in sample 0.33 μg/ml).