CD83 antibody

• Catalog Number: orb545709
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD83
• Clonality: Monoclonal
• Clone Number: HB15e
• Tested applications: FC, IHC-Fr
• Reactivity: Human, Primate
• Isotype: Mouse IgG1 kappa
• Immunogen: Human CD83-transfected Cos cells
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry:Recommended dilution: 1-4 µg/mL, Positive control: peripheral blood-derived dendritic cells. Immunohistochemistry (frozen sections): acetone fixation.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD83
• Entrez: 9308
• UniProt ID: Q01151
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-BL11 antibody, anti-HB15 antibody
• Note: For research use only
• Application notes: Flow cytometry:Recommended dilution: 1-4 µg/mL, Positive control: peripheral blood-derived dendritic cells. Immunohistochemistry (frozen sections): acetone fixation.
• Expiration Date: 12 months from date of receipt.

Separation of IFN alpha-stimulated human monocytes (red) from unstimulated peripheral blood mononuclear cells (black) stained using anti-human CD83 (HB15e) purified antibody (concentration in sample 2 μg/ml, GAM APC) in flow cytometry analysis (surface staining).