CD79b Antibody

• Catalog Number: orb215923
• Category: Antibodies
• Description: CD79b Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CD79b recombinant protein (Position: A29-E229). Human CD79b shares 70% amino acid (aa) sequence identity with mouse CD79b.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 26048 MW
• UniProt ID: P40259
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: B-cell antigen receptor complex-associated protein
• Note: For research use only
• Application notes: WB: The detection limit for CD79b is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of CD79B using anti-CD79B antibody.Lane 1:recombinant human CD79B protein 0.5 ng.

WB analysis of CD79B using anti-CD79B antibody.Lane 1:human Raji cell; 2:human Hl-60 cell; 3:human HUT cell.

IF analysis of CD79B using anti-CD79B antibody.CD79B was detected in immunocytochemical section of A431 cell.

IF analysis of CD79B using anti-CD79B antibody.CD79B was detected in immunocytochemical section of A431 cell.

IHC analysis of CD79B using anti-CD79B antibody. CD79B was detected in a paraffin-embedded section of mouse spleen tissue.

IHC analysis of CD79B using anti-CD79B antibody. CD79B was detected in a paraffin-embedded section of rat spleen tissue.

IHC analysis of CD79B using anti-CD79B antibody. CD79B was detected in a paraffin-embedded section of human tonsil tissue.