CD79a antibody

• Catalog Number: orb154414
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD79a which expressed in B-cells. Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3). The cytoplasmic tails of CD79A contains an ITAM (immuno-receptor tyrosine-based activation) motif, which acts to initiate the BCR signaling reactions by binding to and activating tyrosine
• Clonality: Monoclonal
• Clone Number: HM47
• Tested applications: FC, IHC-P, IP, WB
• Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Human, Mouse, Porcine, Primate, Rabbit, Rat
• Isotype: Mouse IgG1 kappa
• Immunogen: Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml, intracellular staining.Western blotting: Recommended dilution: 1-2 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD79a
• Entrez: 973
• UniProt ID: P11912
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-BCR alpha antibody, anti-Ig-alpha antibody, a
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Separation of human CD79a positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human CD79a (HM47) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human CD79a (HM47) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Western blotting analysis of human CD79a using mouse monoclonal antibody HM47 on lysates of Raji and Jurkat (negative control) cell line under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-H-Ras monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD79a was detected around 43 kDa.