CD74 Mouse Monoclonal Antibody

SKU: orb500373

Description

CD74 Mouse Monoclonal Antibody

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: B0F4
• Immunogen: KLH conjugated synthetic peptide derived from human CD74 (1-232/296aaaa)
• Target: CD74
• Molecular Weight: 35 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CLIP; DHLAG; HLADG; II; Ia-GAMMA; p33; HG2A_HUMAN; CD74; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain (Ii); HG2A_MOUSE; Ia antigen-associated invariant chain (Ii | Ii chain); MHC class II-associated invariant chain; CD74 molecule; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; HLA-DR-gamma; Ia-associated invariant chain; gamma chain of class II antigens; MHC HLA-DR gamma chain; class II MHC-associated invariant chain peptide

Paraformaldehyde-fixed, paraffin embedded (Human appendix), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD74) Monoclonal Antibody, Unconjugated (orb500373) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD74) Monoclonal Antibody, Unconjugated (orb500373) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD74) Monoclonal Antibody, Unconjugated (orb500373) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Quick Database Links

Gene Symbol

CD74

UniProt

P04233