CD74 Antibody

• Catalog Number: orb1671617
• Category: Antibodies
• Description: CD74 Antibody
• Clonality: Recombinant
• Clone Number: LN-2
• Tested applications: ELISA, FC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunizing mice with SU-DHL-4 lymphoma cells.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CD74
• UniProt ID: P04233
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Ia antigen, HLA class II histocompatibility antige
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed Daudi cells with anti-CD74 antibody LN-2. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric r version of LN-2 (orb1671617) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671617- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD74 antibody LN-2. Daudi cell lysate (A)- human spleen (B) and human tonsil (C) tissue lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of LN-2 (orb1671617) at 0.0001 ug/ml- 0.001 ug/ml and 0.0001 ug/ml- respectively- before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow cytometry using the Anti-CD74 antibody LN-2. Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of LN-2 (orb1671617 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.