CD74 Antibody

• Catalog Number: orb1251985
• Category: Antibodies
• Description: CD74 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CDLA74-1
• Tested applications: FC, IF, IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant full-length human protein was used as the immunogen for the CD74 antibody. (3)
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry: 1-2 ug/ml (1)Prediluted format : incubate for 30 min at RT (2)Titering of the CD74 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. The CD74 antibody epitope is known to be extracellular.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD74
• UniProt ID: P04233
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: CD74, DHLAG, HLADG, Ia-GAMMA, CD74 molecule, major
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry: 1-2 ug/ml (1)Prediluted format : incubate for 30 min at RT (2)Titering of the CD74 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. The CD74 antibody epitope is known to be extracellular.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human tonsil with CD74 antibody (clone CDLA74-1).