CD74 Antibody

• Catalog Number: orb1147813
• Category: Antibodies
• Description: CD74 (the MHC II-associated invariant chain, Ii) is a type II transmembrane protein expressed in antigen-presenting cells, that serves as MHC II chaperone which promotes MHC II trafficking from the ER to endocytic compartments, prevents peptide binding in the ER and contributes to peptide editing in the MHC II compartment; it is also an accessory signaling molecule implicated e.g. in malignant B cell proliferation. Stimulation of cell surface CD74 leads to NFkappaB activation, which enables entry of the stimulated cell into the S phase. CD74 binds pro-inflammatory cytokine MIF with high affinity and interacts with CD44. Binding of Vpu, an HIV1 protein, to CD74 modulates MHC II presentation.
• Clonality: Monoclonal
• Clone Number: LN2
• Tested applications: FC, ICC, IHC-P, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: SU-DHL-4 lymphoma cells
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 2-5 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD74
• Entrez: 972
• UniProt ID: P04233
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: p33, DHLAG, HLADG, Ia-gamma
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 2-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of human CD74 positive lymphocytes (red-filled) from CD74 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD74 (LN2) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD74 (LN2) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).