CD74 Antibody [In-1], Rabbit IgG

• Catalog Number: orb1671845
• Category: Antibodies
• Description: CD74 Antibody [In-1], Rabbit IgG
• Clonality: Recombinant
• Clone Number: In-1
• Tested applications: FC, IHC-Fr, IP
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: Mouse lymphocytes of strain A injected into Wistar rats.
• Concentration: batch dependent
• Conjugation: Unconjugated
• UniProt ID: CD74
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Ia antigen, DHLAG, HLADG, Ia-gamma, H-2 class II h
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed mouse splenocytes with anti-CD74 antibody In-1. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version of In-1 (orb1671845) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671845- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD74 antibody In-1. Mouse spleen (A) and mouse lymph node (B) lysate samples (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of In-1 (orb1671845) at 0.0001 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD74 is 31.6kDa- and this protein is glycosylated at several residues. orb1671845 successfully detected CD74 in mouse spleen and mouse lymph node lysates.