CD7 Antibody

• Catalog Number: orb1672110
• Category: Antibodies
• Description: CD7 Antibody
• Clonality: Recombinant
• Clone Number: 3A1E
• Tested applications: FA, FC
• Reactivity: Human
• Isotype: IgG kappa
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CD7
• UniProt ID: P09564
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Leu9, T-cell antigen CD7, GP40, T-cell leukemia an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed Jurkat cells with anti-CD7 antibody 3A1E. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version of 3A1E (orb1672110) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672110- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD7 antibody 3A1E. Jurkat cell lysate samples (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of 3A1E (orb1672110) at 1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD7 is 25.4kDa- but this protein is glycosylated at several residues. orb1672110 successfully detected CD7 in Jurkat cell lysate.